Journal: Animal Models and Experimental Medicine
Article Title: Bioinformatics‐based discovery of the involvement of PSAT1 in mediating the anti‐lung adenocarcinoma activity of triptolide
doi: 10.1002/ame2.70120
Figure Lengend Snippet: Detection of PSAT1 expression and its correlation analysis with clinical characteristics. (A) Pan‐cancer analysis of the expression of PSAT1 in the TIMER database. (B) The expression of PSAT1 mRNA in LUAD tissues in the TCGA database. (C) The expression of PSAT1 mRNA in paired tumor tissues and adjacent normal tissues in the TCGA database. (D–G) The clinical correlations between the expression of PSAT1 and age, gender, stage, and lymph node metastasis. (H) The immunohistochemical results of PSAT1 in the tumor tissues of tumor‐bearing mice. (I) The results of quantitative analysis of immunohistochemistry ( n = 3). (J) Representative PSAT1 immunofluorescence results in A549 cells. (K) PSAT1 relative fluorescence intensity ( n = 5). * p < 0.05, ** p < 0.01, *** p < 0.001.
Article Snippet: Sections were incubated with PSAT1 primary antibody (Proteintech) overnight at 4°C, washed, and then incubated with secondary antibody.
Techniques: Expressing, Immunohistochemical staining, Immunohistochemistry, Immunofluorescence, Fluorescence